Neuromuscular DiseasesNeuromuscular DiseasesНервно-мышечные болезни2222-87212413-0443Publishing House ABV Press3910.17650/2222-8721-2013-0-1-35-45ORIGINAL REPORTSОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯDirect morphological identification of Borrelia burgdorferi in the muscle biopsies: the possibility of association of the neuromuscular abnormalities with BorreliosisПрямая морфологическая детекция Borrelia burgdorferi в мышечных биоптатах: возможность связи нервно-мышечной патологии с боррелиозомSakharovaA. V.СахароваА. В.
Russian Federation
alla.sakharova@gmail.comDidenkoL. V.ДиденкоЛ. В.
Russian Federation
MuravinaT. I.МуравинаТ. И.
Russian Federation
ChaikovskayaR. P.ЧайковскаяР. П.
Russian Federation
KostE. A.КостЕ. А.
Russian Federation
Mir-KasimovM. F.Мир-КасимовМ. Ф.
Russian Federation
Research Institute of Neurology, Russian Academy of Medical SciencesФГБУ «Научный центр неврологии» РАМНN.F. Gamaleya Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, MoscowФГБУ «Институт эпидемиологии и микробиологии им. Н.Ф. Гамалеи» Минздрава России, Москва190520133135451902201519022015Copyright ©; 2013, Sakharova A.V., Didenko L.V., Muravina T.I., Chaikovskaya R.P., Kost E.A., Mir-Kasimov M.F.Copyright ©; 2013, Сахарова А.В., Диденко Л.В., Муравина Т.И., Чайковская Р.П., Кост Е.А., Мир-Касимов М.Ф.2013Sakharova A.V., Didenko L.V., Muravina T.I., Chaikovskaya R.P., Kost E.A., Mir-Kasimov M.F.Сахарова А.В., Диденко Л.В., Муравина Т.И., Чайковская Р.П., Кост Е.А., Мир-Касимов М.Ф.https://creativecommons.org/licenses/by/4.0https://nmb.abvpress.ru/jour/article/view/39

The authors examined 40 muscle biopsy specimens taken from patients with neuromuscular symptoms when the diagnosis was unestablished or presumptive. Eighteen of them exhibited foci of muscle fiber damage with the presence of spirochete-like structures in the semithin tissue sections. Electron microscopy of these areas detected Borrelia as vegetative and diverse L-forms. Immunocytochemical techniques usingantibodies to Borrelia burgdorferi antigens confirmed that the spirochetes belonged to this species. This allows one to consider borreliosis as an etiological or complicating factor of neuromuscular pathology and to recommend the above morphological methods for the diagnosis of neuromuscular diseases of unknown origin.

Исследовано 40 биоптатов мышц, взятых у больных с нервно-мышечной симптоматикой при неустановленном или предположительном диагнозе. В 18 из них в полутонких срезах ткани обнаружены очаги повреждения мышечных волокон с наличием спирохетоподобных структур. При электронной микроскопии этих участков были выявлены боррелии в виде вегетативных и разнообразных L-форм. Применение методов иммуноцитохимии с антителами к антигенам Borrelia burgdorferi подтвердило принадлежность спирохет к данному виду. Это позволило считать этиологическим или осложняющим фактором нервно-мышечной патологии боррелиоз, а также рекомендовать применять указанные морфологические методы как диагностические в случаях нервно-мышечных заболеваний неясного происхождения.

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